Antisense PNA tridecamers targeted to the coding region of ha- ras mRNA arrest polypeptide chain elongation
Identifieur interne : 000268 ( France/Analysis ); précédent : 000267; suivant : 000269Antisense PNA tridecamers targeted to the coding region of ha- ras mRNA arrest polypeptide chain elongation
Auteurs : Nathalie Dias [France] ; Sonia Dheur [France] ; Peter E. Nielsen [Danemark] ; Sergei Gryaznov [États-Unis] ; Arthur Van Aerschot [Belgique] ; Piet Herdewijn [Belgique] ; Claude Hélène [France] ; Tula E. Saison-Behmoaras [France]Source :
- Journal of Molecular Biology [ 0022-2836 ] ; 1999.
English descriptors
- KwdEn :
- Teeft :
- Antisense, Antisense activity, Antisense agents, Antisense oligonucleotides, Arrest translation, Assay, Biol, Cellular proteins, Central mismatch, Chem, Coding, Coding region, Codon, Codon region, Denaturing, Duplex, Elongating ribosomes, Elongation, Entire coding region, Gene expression, Hybrid, Hybridization, Initiation codon, Knudsen nielsen, Lysate, Migration position, Mismatch, Molecular dynamics phosphorimager, Mrna, Mrna translation, Natl acad, Nielsen, Nucl, Oligonucleotide, Oligonucleotides, Peptide, Phenol extraction, Phosphoramidates, Pnas, Polynucleotide kinase, Present study, Primer, Rabbit reticulocyte lysate, Receptor, Reticulocyte, Ribosome, Rnase, Stable duplexes, Transcriptase, Transcription, Translation, Translation assay, Translation initiation, Translation initiation site, Translation machinery, Translation medium, Tridecamer, Tridecamers, Urea.
Abstract
Abstract: We have previously described the rational design of mutation-selective antisense oligonucleotides targeted to codon 12 of oncogenic Ha-ras mRNA. In order to further improve the biological efficacy of these unmodified oligonucleotides, we have studied three different classes of modifications: peptide nucleic acid backbone (PNA), sugar modification (2′-O-methyl) and phosphoramidate linkage (PN). We show that PNA is unique among the investigated steric blocking agents in its ability to specifically inhibit the translation of Ha-ras mRNA in vitro. The PNA-RNA hybrid (Tm=86 °C), which is not dissociated by cellular proteins and resists phenol extraction and urea denaturing conditions, specifically blocks the translation of mutated Ha-ras mRNA. A PNA tridecamer which forms with wild-type Ha-ras mRNA a duplex with a central mismatch had little effect on mRNA translation. Codon 12 is located close to the translation initiation site and hybridization of the PNA at this position may interfere with the assembly of the translation initiation complex. To test whether polypeptide chain elongation can also be blocked, we have targeted PNA tridecamers to codons in the 74, 128 and 149 regions. These PNAs form equally stable duplexes as that formed by the PNA targeted to the codon 12 region (ten G·C base-pairs out of 13). We show that PNA-RNA duplexes block the progression of the 80 S ribosome. Therefore, it is possible to arrest translation with concomitant production of a truncated protein by using duplex-forming PNA oligonucleotides targeted to a G+C-rich sequences. Our data demonstrate for the first time that a non-covalent duplex can arrest the translation machinery and polypeptide chain elongation.
Url:
DOI: 10.1006/jmbi.1999.3277
Affiliations:
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Saison-Behmoaras</name><affiliation wicri:level="1"><country wicri:rule="url">France</country></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biophysique, Muséum National d’Histoire Naturelle, INSERM U201 CNRS UMR, 8646 43 rue Cuvier 75231</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Molecular Biology</title><title level="j" type="abbrev">YJMBI</title><idno type="ISSN">0022-2836</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">294</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="403">403</biblScope><biblScope unit="page" to="416">416</biblScope></imprint><idno type="ISSN">0022-2836</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-2836</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ha-ras mRNA</term><term>PN</term><term>PNA</term><term>PNA/RNA duplexes</term><term>RRL</term><term>affinity of interaction</term><term>antisense</term><term>translation elongation arrest</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Antisense</term><term>Antisense activity</term><term>Antisense agents</term><term>Antisense oligonucleotides</term><term>Arrest translation</term><term>Assay</term><term>Biol</term><term>Cellular proteins</term><term>Central mismatch</term><term>Chem</term><term>Coding</term><term>Coding region</term><term>Codon</term><term>Codon region</term><term>Denaturing</term><term>Duplex</term><term>Elongating ribosomes</term><term>Elongation</term><term>Entire coding region</term><term>Gene expression</term><term>Hybrid</term><term>Hybridization</term><term>Initiation codon</term><term>Knudsen nielsen</term><term>Lysate</term><term>Migration position</term><term>Mismatch</term><term>Molecular dynamics phosphorimager</term><term>Mrna</term><term>Mrna translation</term><term>Natl acad</term><term>Nielsen</term><term>Nucl</term><term>Oligonucleotide</term><term>Oligonucleotides</term><term>Peptide</term><term>Phenol extraction</term><term>Phosphoramidates</term><term>Pnas</term><term>Polynucleotide kinase</term><term>Present study</term><term>Primer</term><term>Rabbit reticulocyte lysate</term><term>Receptor</term><term>Reticulocyte</term><term>Ribosome</term><term>Rnase</term><term>Stable duplexes</term><term>Transcriptase</term><term>Transcription</term><term>Translation</term><term>Translation assay</term><term>Translation initiation</term><term>Translation initiation site</term><term>Translation machinery</term><term>Translation medium</term><term>Tridecamer</term><term>Tridecamers</term><term>Urea</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We have previously described the rational design of mutation-selective antisense oligonucleotides targeted to codon 12 of oncogenic Ha-ras mRNA. In order to further improve the biological efficacy of these unmodified oligonucleotides, we have studied three different classes of modifications: peptide nucleic acid backbone (PNA), sugar modification (2′-O-methyl) and phosphoramidate linkage (PN). We show that PNA is unique among the investigated steric blocking agents in its ability to specifically inhibit the translation of Ha-ras mRNA in vitro. The PNA-RNA hybrid (Tm=86 °C), which is not dissociated by cellular proteins and resists phenol extraction and urea denaturing conditions, specifically blocks the translation of mutated Ha-ras mRNA. A PNA tridecamer which forms with wild-type Ha-ras mRNA a duplex with a central mismatch had little effect on mRNA translation. Codon 12 is located close to the translation initiation site and hybridization of the PNA at this position may interfere with the assembly of the translation initiation complex. To test whether polypeptide chain elongation can also be blocked, we have targeted PNA tridecamers to codons in the 74, 128 and 149 regions. These PNAs form equally stable duplexes as that formed by the PNA targeted to the codon 12 region (ten G·C base-pairs out of 13). We show that PNA-RNA duplexes block the progression of the 80 S ribosome. Therefore, it is possible to arrest translation with concomitant production of a truncated protein by using duplex-forming PNA oligonucleotides targeted to a G+C-rich sequences. Our data demonstrate for the first time that a non-covalent duplex can arrest the translation machinery and polypeptide chain elongation.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>Danemark</li><li>France</li><li>États-Unis</li></country><region><li>Île-de-France</li></region></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Dias, Nathalie" sort="Dias, Nathalie" uniqKey="Dias N" first="Nathalie" last="Dias">Nathalie Dias</name></region><name sortKey="Dheur, Sonia" sort="Dheur, Sonia" uniqKey="Dheur S" first="Sonia" last="Dheur">Sonia Dheur</name><name sortKey="Helene, Claude" sort="Helene, Claude" uniqKey="Helene C" first="Claude" last="Hélène">Claude Hélène</name><name sortKey="Saison Behmoaras, Tula E" sort="Saison Behmoaras, Tula E" uniqKey="Saison Behmoaras T" first="Tula E" last="Saison-Behmoaras">Tula E. Saison-Behmoaras</name><name sortKey="Saison Behmoaras, Tula E" sort="Saison Behmoaras, Tula E" uniqKey="Saison Behmoaras T" first="Tula E" last="Saison-Behmoaras">Tula E. Saison-Behmoaras</name></country><country name="Danemark"><noRegion><name sortKey="Nielsen, Peter E" sort="Nielsen, Peter E" uniqKey="Nielsen P" first="Peter E" last="Nielsen">Peter E. Nielsen</name></noRegion></country><country name="États-Unis"><noRegion><name sortKey="Gryaznov, Sergei" sort="Gryaznov, Sergei" uniqKey="Gryaznov S" first="Sergei" last="Gryaznov">Sergei Gryaznov</name></noRegion></country><country name="Belgique"><noRegion><name sortKey="Van Aerschot, Arthur" sort="Van Aerschot, Arthur" uniqKey="Van Aerschot A" first="Arthur" last="Van Aerschot">Arthur Van Aerschot</name></noRegion><name sortKey="Herdewijn, Piet" sort="Herdewijn, Piet" uniqKey="Herdewijn P" first="Piet" last="Herdewijn">Piet Herdewijn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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